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Year : 2005  |  Volume : 23  |  Issue : 3  |  Page : 182-185

Sunflower seed husk agar: A new medium for the differentiation of Candida dubliniensis from Candida albicans


1 Department of Microbiology, Faculty of Medicine, Kuwait University, PO Box 24923, 13110, Kuwait
2 Department of Microbiology, Faculty of Medicine, Kuwait University, PO Box 24923, 13110

Correspondence Address:
Z U Khan
Department of Microbiology, Faculty of Medicine, Kuwait University, PO Box 24923, 13110
Kuwait
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.16605

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A sunflower ( Helianthus annuus ) seed husk agar medium has been developed and evaluated for differentiation of Candida dubliniensis from Candida albicans on the basis of colony morphology and chlamydospore production. All C. dubliniensis isolates (n=40) produced rough colonies with hyphal fringes and abundant chlamydospores whereas 101 of 105 (96.2%) C. albicans isolates produced smooth colonies with no evidence of chlamydospore production. Since this medium is free from oil droplets, chlamydospores can be examined with greater clarity by Dalmau plate technique. This medium provides a simple and cost-effective tool for the presumptive differentiation of C. dubliniensis from C. albicans and is particularly suited for clinical microbiology laboratories where biochemical or molecular methods for the differentiation of these two species are not available.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04