Indian Journal of Medical Microbiology IAMM  | About us |  Subscription |  e-Alerts  | Feedback |  Login   
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size
 Home | Ahead of Print | Current Issue | Archives | Search | Instructions  
Users Online: 859 Official Publication of Indian Association of Medical Microbiologists 
 ~   Next article
 ~   Previous article
 ~   Table of Contents

 ~   Similar in PUBMED
 ~  Search Pubmed for
 ~  Search in Google Scholar for
 ~Related articles
 ~   Citation Manager
 ~   Access Statistics
 ~   Reader Comments
 ~   Email Alert *
 ~   Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed10110    
    Printed348    
    Emailed24    
    PDF Downloaded704    
    Comments [Add]    
    Cited by others 3    

Recommend this journal

 

 BRIEF COMMUNICATIONS
Year : 2005  |  Volume : 23  |  Issue : 1  |  Page : 52-55

Detection of vancomycin resistant Staphylococcus aureus: A comparative study of three different phenotypic screening methods


1 Department of Microbiology, Ranbaxy Research Laboratories, R&D II, Sector 18, Udyog Vihar Industrial Area, Gurgaon-122 001, Haryana, India
2 Jamia Millia Islamia University, New Delhi, India

Correspondence Address:
Ashok Rattan
Department of Microbiology, Ranbaxy Research Laboratories, R&D II, Sector 18, Udyog Vihar Industrial Area, Gurgaon-122 001, Haryana
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.13875

Rights and Permissions

The objective of this study was to investigate screening methodologies, to detect Staphylococcus aureus strains with decreased susceptibility to vancomycin. Three methods were used to screen 160 Staphylococcus aureus clinical isolates along with ATCC quality control strains. Subsequently, MIC of all these 160 strains were determined by NCCLS methodology. The MIC of all the 160 clinical isolates was < 4µg/mL and were classified as vancomycin susceptible by NCCLS criteria but 23 strains were positive by Hiramatshu method, two grew on MHA (5µg/mL vancomycin) while CDC method correctly identified no vancomycin intermediate S.aureus (VISA) or vancomycin resistant S.aureus (VRSA) strains with reference to there MIC. CDC method was found to be the most appropriate screening methodology for detection of VISA or VRSA for diagnostic laboratories.






[FULL TEXT] [PDF]*


        
Print this article     Email this article

2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04