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 ORIGINAL ARTICLE
Year : 2005  |  Volume : 23  |  Issue : 1  |  Page : 29-33

Comparison of the conventional diagnostic modalities, bactec culture and polymerase chain reaction test for diagnosis of tuberculosis


1 Department of Microbiology, National Institute of Communicable Diseases, New Delhi - 110 054, India
2 Department of Biosciences, National Institute of Communicable Diseases, New Delhi - 110 054, India
3 Department of Biochemistry and Biotechnology, National Institute of Communicable Diseases, New Delhi - 110 054, India

Correspondence Address:
Sunil Gupta
Department of Microbiology, National Institute of Communicable Diseases, New Delhi - 110 054
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.13869

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PURPOSE: To evaluate the performance of 65 kDa antigen based PCR assay in clinical samples obtained from pulmonary and extrapulmonary cases of tuberculosis. METHODS: One hundred and fifty six samples were processed for detection of Mycobacterium tuberculosis by ZN smear examination, LJ medium culture, BACTEC radiometric culture and PCR tests. RESULTS: A significant difference was seen in the sensitivities of different tests, the figures being 74.4% for PCR test, 33.79% for ZN smear examination, 48.9% for LJ culture and 55.8% for BACTEC culture (P<0.05). However, there was no significant difference (P>0.05) as far as specificity of different tests was concerned. PCR test sensitivity in pulmonary and extrapulmonary clinical samples were 72.7% and 75.9% respectively and found to be significantly higher (P<0.05) when compared with those of other tests. The mean detection time for M.tuberculosis was 24.03 days by LJ medium culture, 12.89 days by BACTEC culture and less than one day by PCR test. CONCLUSIONS: PCR is a rapid and sensitive method for the early diagnosis of pulmonary and extrapulmonary tuberculosis.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04