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Year : 2004  |  Volume : 22  |  Issue : 4  |  Page : 247-250

Detection of -Lactamases in nosocomial gram negative clinical isolates

Department of Microbiology, P. D. Hinduja National Hospital and Medical Research Center, Mahim, Mumbai - 400 016, Maharashtra, India

Correspondence Address:
C Rodrigues
Department of Microbiology, P. D. Hinduja National Hospital and Medical Research Center, Mahim, Mumbai - 400 016, Maharashtra
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Source of Support: None, Conflict of Interest: None

PMID: 17642748

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-lactamases represent the most common mechanism of -lactam resistance. Extended spectrum -lactamases (ESBLs) represent a major group of -lactamses currently being identified worldwide in large numbers along with inducible AmpC -lactamases and derepressed mutants. The present study was done to detect -lactamase production in clinical isolates by rearranging routine discs used in reporting susceptibility to specifically assess ESBLs, AmpC -lactamases (both inducible and hyperproducers i.e., derepressed mutants). A total of 286 clinical isolates were studied using a novel predictor disc approximation method to detect the above mechanisms of resistance with careful use and placement of antimicrobial discs. Of the 286 isolates, 151(53%) were ESBL producers of which 131(46%) were also derepressed mutants while remaining 20(7%) were plain ESBL producers. Forty (14%) were plain derepressed mutants. Inducible AmpC -lactamase production was detected in 19(7%) of the isolates. The commonest ESBL producers were E.coli and K. pneumoniae. The high incidence of -lactamase production due to multiple mechanisms in clinical isolates is alarming and urgent action needs to be taken from both a therapeutic and infection control perspective.


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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04