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Year : 2004  |  Volume : 22  |  Issue : 3  |  Page : 169-171

Relative susceptibility of six continuous cell lines for cultivation of chlamydia trachomatis strains


L & T Microbiology Research Center, Vision Research Foundation, Sankara Nethralaya, Chennai - 600 006, Tamil Nadu, India

Correspondence Address:
H N Madhavan
L & T Microbiology Research Center, Vision Research Foundation, Sankara Nethralaya, Chennai - 600 006, Tamil Nadu
India
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Source of Support: None, Conflict of Interest: None


PMID: 17642725

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Since susceptibility of a cell line is an important factor for cultivation of Chlamydia trachomatis, McCoy, HeLa, BHK-21, HEp-2, Vero and A549 cell lines were tested for this characteristic. These were inoculated with 150 infection-forming units (IFU) of C. trachomatis A, B, Ba and C serovars. Growth was graded according to the number of IFUs per microscopic field (100X). A549-cell line was not susceptible to infection by any of the serovars. The growth of C. trachomatis was good to very good in McCoy and HeLa cell lines. Vero, BHK-21 and HEp-2 cell lines varied considerably in the susceptibility to infection.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04