Indian Journal of Medical Microbiology IAMM  | About us |  Subscription |  e-Alerts  | Feedback |  Login   
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size
 Home | Ahead of Print | Current Issue | Archives | Search | Instructions  
Users Online: 679 Official Publication of Indian Association of Medical Microbiologists 
  Search
 
 ~ Next article
 ~ Previous article 
 ~ Table of Contents
  
 ~  Similar in PUBMED
 ~  Search Pubmed for
 ~  Search in Google Scholar for
 ~  Article in PDF (11 KB)
 ~  Citation Manager
 ~  Access Statistics
 ~  Reader Comments
 ~  Email Alert *
 ~  Add to My List *
* Registration required (free)  

 
 ~  References

 Article Access Statistics
    Viewed4880    
    Printed147    
    Emailed4    
    PDF Downloaded237    
    Comments [Add]    

Recommend this journal

 
CORRESPONDENCE
Year : 2004  |  Volume : 22  |  Issue : 1  |  Page : 68
 

Evaluation of the direct acridine orange staining method for diagnosis of malaria


Department of Microbiology, University College of Medical Sciences, New Delhi - 110 095, India

Correspondence Address:
Department of Microbiology, University College of Medical Sciences, New Delhi - 110 095, India



How to cite this article:
Nandwani S. Evaluation of the direct acridine orange staining method for diagnosis of malaria. Indian J Med Microbiol 2004;22:68


How to cite this URL:
Nandwani S. Evaluation of the direct acridine orange staining method for diagnosis of malaria. Indian J Med Microbiol [serial online] 2004 [cited 2019 Aug 25];22:68. Available from: http://www.ijmm.org/text.asp?2004/22/1/68/8070


Dear Editor,
Malaria causes 300 to 500 million infections and 1.5 to 2.7 million deaths each year worldwide.[1] Conventional Giemsa stained peripheral blood smear examination remains the gold standard for diagnosis of malaria in malaria endemic countries. However, the technique is labour intensive time consuming and may give poor results in cases with low parasitaemia.[2] In recent years numerous quick and new techniques for malaria diagnosis have been developed, one such being direct AO (acridine orange) staining technique, which was evaluated in this study.
A total of 310 blood samples were selected from patients presenting with pyrexia and/or atypical presentations. Giemsa and AO staining of peripheral blood smears for diagnosis of malaria were done simultaneously on all blood samples. AO staining was found to be 93.3% sensitive and 99.25% specific. Out of the 45 Giemsa smear positive cases (29 Plasmodium vivax, 15 Plasmodium falciparum and 1 mixed) AO staining was able to detect 42 cases. Out of the 265 Giemsa smear negative cases 2 were positive by AO staining. Comparing the examination of ordinary Giemsa thin blood film with the use of thick films, the thin film examination had a sensitivity of 71.1% and a specificity of 100%.
Our results demonstrated a higher sensitivity and rapidity of AO staining as compared to Giemsa stained thin blood films, confirming the results of other field studies.[3] Species differentiation was quite reliable with AO staining. It required only 10 minutes for reporting even at a low parasitaemia by AO staining method. Cost of each smear by AO was Rs.4 -5 per smear. We conclude that in terms of performance, speed, ease of use and reliability, the AO method would be ideal to support diagnosis of malaria. However, the cost of the microscope and special accessories need to be considered. 

 ~ References Top

1.World Health Organization. World malaria situation in 1994. Wkly Epidemiol Rec 1997;36:269-276; No. 37: 277-284; No.38, 285-292.  Back to cited text no. 1    
2.Craig MH, Sharp BL. Comparative evaluation of four techniques for the diagnosis of P. falciparum infections. Trans R Soc Trop Med Hyg 1997;91:279-282.  Back to cited text no. 2    
3.Gay F, Traore' B, Zanoni J, et al. Direct acridine orange fluorescence examination of blood slides compared to current techniques for malaria diagnosis. Trans R Soc Trop Med Hyg 1996;90:516-518.  Back to cited text no. 3    
Top
Print this article  Email this article
Previous article Next article

    

2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04