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 ORIGINAL ARTICLE
Year : 2003  |  Volume : 21  |  Issue : 1  |  Page : 17-20

Search for shiga toxin producing escherichia coli (STEC) including 0157: H7 strains in and around Kolkata


1 Department of Microbiology, All India Institute of Hygiene & Public Health, Kolkata - 700 073, India
2 Division of Microbiology, National Institute of Cholera and Enteric Diseases, Kolkata - 700 010, India

Correspondence Address:
U K Chattopadhyay
All India Institute of Hygiene & Public Health, Kolkata - 700 073
India
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Source of Support: None, Conflict of Interest: None


PMID: 17642968

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PURPOSE: An attempt was made to isolate and characterize Shiga toxin producing E. coli (STEC) from animals handlers, animal products and admitted diarrhoeic children in and around Kolkata. METHODS: A total of 415 samples were processed for detection of STEC by PCR and colony hybridization techniques. RESULTS: 0 (4.81%) samples were found to be positive for STEC. Diarrhoeic cattle accounted for maximum (22.1 %) isolation. CONCLUSIONS: The study showed PCR to be more sensitive than hybridization technique for detection of STEC.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04