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 ORIGINAL ARTICLE
Year : 2002  |  Volume : 20  |  Issue : 3  |  Page : 137-140

A preliminary report on characterization and identification of non tuberculous mycobacteria (NTM) on the basis of biochemical tests and protein / isoenzyme electrophoretic patterns


Central JALMA Institute for Leprosy (ICMR), Tajganj, Agra - 282 001, India

Correspondence Address:
V D Sharma
Central JALMA Institute for Leprosy (ICMR), Tajganj, Agra - 282 001
India
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Source of Support: None, Conflict of Interest: None


PMID: 17657052

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PURPOSE: To assess the usefulness of protein electrophorograms and protein zymodemes in the identification and characterization of non tuberculous mycobacteria (NTM). METHODS: Cell free extracts (CFEs) from 22 mycobacterial isolates belonging to slow growing and other clinically relevant species were included in the study. The strains isolated from the environment were identified on the basis of their standard biochemical tests; pigmentation and growth characters. The CFEs were electrophoresed and stained for proteins and esterases. RESULTS: Most of the isolates identified on the basis of biochemical tests exhibited characteristic protein and esterase pattern for M.scrofulaceum, M.avium and M.xenopi. Others showed variations in their proteins and esterase pattern though they were identified as M.scrofulaceum, M.avium and M.xenopi. CONCLUSIONS: Based on these studies it appears that because of variability in the protein and isoenzyme patterns of NTM, it may be advisable to use them along with biochemical tests and other tests for identifying and characterizing the different mycobacterial species belonging to slow growers.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04