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Year : 2000  |  Volume : 18  |  Issue : 2  |  Page : 77-78

Growth of Mycobacterium tuberculosis an modified thayer martin medium

1 Department of Microbiology, LT. MM College, Sion Mumbai 400 022

Correspondence Address:
S Vishwanath
Department of Microbiology, LT. MM College, Sion Mumbai 400 022

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Source of Support: None, Conflict of Interest: None

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ABSTRACT: 25 sputum samples from patients with pulmonary tuberculosis positive for acid fast bacilli on smear were inoculated on Modified Thayer Martin Medium (MTM) with antibiotic supplement of vancomycin (7.5 miu g/ml), colistin (4 miu g/ml). nystatin (12,500 miu g/ml) and 5 percent lysed human blood. No concentration procedure was performed prior to inoculation. Lowenstein Jensen (LJ) medium was also inoculated after concentration of the specimens by Petroff's method. The growth of Mycobacterium tuberculosis was seen in 10-12 days on MTM as compared to the growth on LJ medium which was seen at 3 weeks. A control strain of H37Rv of M.tuberculosis inoculated on both media showed similar results. The preliminary finding that M.tuberculosis grows on Modified Thayer Martin medium is being reported.

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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04