Indian Journal of Medical Microbiology IAMM  | About us |  Subscription |  e-Alerts  | Feedback |  Login   
  Print this page Email this page   Small font sizeDefault font sizeIncrease font size
 Home | Ahead of Print | Current Issue | Archives | Search | Instructions  
Users Online: 1623 Official Publication of Indian Association of Medical Microbiologists 
 ~   Next article
 ~   Previous article
 ~   Table of Contents

 ~   Similar in PUBMED
 ~  Search Pubmed for
 ~  Search in Google Scholar for
 ~Related articles
 ~   Citation Manager
 ~   Access Statistics
 ~   Reader Comments
 ~   Email Alert *
 ~   Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded0    
    Comments [Add]    

Recommend this journal


Year : 1999  |  Volume : 17  |  Issue : 4  |  Page : 184-186

Comparison of enzyme immunoassay and direct fluorescent antibody test for the detection of chlamydia trachomatis in non-gonococcal urethritis

Department of Microbiology, Maulana Azad Medical College, New Delhi 110 002

Correspondence Address:
D Nair
Department of Microbiology, Maulana Azad Medical College, New Delhi 110 002

Login to access the Email id

Source of Support: None, Conflict of Interest: None

Rights and PermissionsRights and Permissions

ABSTRACT: The diagnosis of Chlamydia trachomatis infection has been problematic due to the labor-intensive culture techniques. Non-culture techniques made a breakthrough in the diagnosis of this condition. The culture techniques for this pathogen have been standardized in most laboratories, thus non-culture methods have become the mainstay in routine screening this organism. The Direct Fluorescent Antibody test (DFA) and Enzyme Immunoassay (EIA) are the two primary non-culture technique being used frequently. In this study, 124 male STD attendees were screened and 32 were found to suffer from non-gonococcal urethritis. Urethral samples collected from these patients were subjected to DFA and EIA tests. Fourteen samples were positive by DFA and EIA tests each. Both the tests were positive in 12 patient. The EIA test when compared to DFA gave a sensitivity of 75 percent, specificity of 88.0 percent and efficiency of 87.5 percent. Thus the EIA was found to be a cheap and effective screening assay in developing countries.

[PDF Not available]*

Print this article     Email this article

2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04