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Year : 1999  |  Volume : 17  |  Issue : 4  |  Page : 184-186

Comparison of enzyme immunoassay and direct fluorescent antibody test for the detection of chlamydia trachomatis in non-gonococcal urethritis


Department of Microbiology, Maulana Azad Medical College, New Delhi 110 002

Correspondence Address:
D Nair
Department of Microbiology, Maulana Azad Medical College, New Delhi 110 002

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Source of Support: None, Conflict of Interest: None


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ABSTRACT: The diagnosis of Chlamydia trachomatis infection has been problematic due to the labor-intensive culture techniques. Non-culture techniques made a breakthrough in the diagnosis of this condition. The culture techniques for this pathogen have been standardized in most laboratories, thus non-culture methods have become the mainstay in routine screening this organism. The Direct Fluorescent Antibody test (DFA) and Enzyme Immunoassay (EIA) are the two primary non-culture technique being used frequently. In this study, 124 male STD attendees were screened and 32 were found to suffer from non-gonococcal urethritis. Urethral samples collected from these patients were subjected to DFA and EIA tests. Fourteen samples were positive by DFA and EIA tests each. Both the tests were positive in 12 patient. The EIA test when compared to DFA gave a sensitivity of 75 percent, specificity of 88.0 percent and efficiency of 87.5 percent. Thus the EIA was found to be a cheap and effective screening assay in developing countries.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

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