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Year : 1998  |  Volume : 16  |  Issue : 4  |  Page : 149-151

Comparison of DNA amplification and IGM assays for the detection of mycoplasma pneumoniae in primary atypical pneumonia


Department of Microbiology, National Health & Education P.D Hinduja National Hospital & Medical Research Centre Mumbai

Correspondence Address:
C Rodrigues
Department of Microbiology, National Health & Education P.D Hinduja National Hospital & Medical Research Centre Mumbai

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Source of Support: None, Conflict of Interest: None


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ABSTRACT: The aim of this study was to determine the sensitivity of two different methods, DNA amplification and ELISA for the detection of Mycoplasma pneumoniae from clinical samples. Sputum / BAL were obtained from 25 patients and 25 throat swabs from healthy individuals for DNA amplification and serum from all patients and controls for IgM levels respectively. Mpn 38 and Mpn 39 primers were used to amplify a 950 bp fragment of tuf gene in M-pneumoniae. Two patients were positive by ELISA and also for DNA amplification. None of the ELISA positives were negative by DNA amplification and none of the ELISA negatives were positive for M. pneumoniae by DNA amplification. Thus, in a country like India where cost remains a major constraint, ELISA is an adequate and rapid method for the detection of M. pneumoniae.






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2004 - Indian Journal of Medical Microbiology
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