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Year : 1998  |  Volume : 16  |  Issue : 4  |  Page : 138-142

Improving the accuracy of disk diffusion antimicrobial susceptibility testing using species-related interpretive zone break points


Division of clinical Microbiology, Department of Laboratory Medicine, Karolinska Institute/Hospital, 5-17196 STOCKHOLM, SWEDEN, Sweden

Correspondence Address:
G Kronvall
Division of clinical Microbiology, Department of Laboratory Medicine, Karolinska Institute/Hospital, 5-17196 STOCKHOLM, SWEDEN
Sweden
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Source of Support: None, Conflict of Interest: None


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ABSTRACT: Antimicrobial susceptibility testing by the so called quantitative disc diffusion method offers a reproducible test which can be performed in most laboratories. Standardisation of the disc method is essential. In addition, accuracy of the results is improved with the introduction of several species-related exceptions according to recommendations by NCCLS. Further species-related control of the performance in the individual laboratory is recommended using histogram analysis. Major type of interpretive errors in disc diffusion tests are described. For more specific calibration of the disc test, the so called single-strain regression analysis might be employed in collaboration with a reference authority or disc manufacturer.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04