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Year : 1995  |  Volume : 13  |  Issue : 3  |  Page : 119-123

Protein and isoenzyme patterns of mycobacteria II, their role in identification of rapidly growing mycobacteria


Dept. of Microbiology, Central JALMA Institute for leprosy (ICMR), Taj Gang, Agra

Correspondence Address:
VD Sharma
Dept. of Microbiology, Central JALMA Institute for leprosy (ICMR), Taj Gang, Agra

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Source of Support: None, Conflict of Interest: None


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ABSTRACT: Cell free extracts (CFEs) from several slow growing mycobacteria namely m.asiaticum, M.avium, M.gastri, M.gordonae, M.intracellulare, M.kansasii, M.microti, M.nonchromogenicum, M.scrofulaceum, M.simiae, M.szulguai, M.triviale, M.tuberculosis, M.bovis BCG and M.xenopi were electrophoreses and stained for protein as well as lactate dehydrogenase (LDH), esterase, malate dehydrogenase (MDH), malic enzyme ME), alanine dehydrogenase (Alanine DH), and superoxide dismutase (SOD), alcohol dehydrogenase (Alcohol DH), and succinic dehydrogenase activity. It was observed that a combination of the protein and LDH produced clearly distinguishable patterns for all the mycobacteria included in the study. MDH and ME patterns were identified as additional useful taxonomic markers for identification of various slow growing nonchromogens and photochromogens. Identification could be completed within one working day after obtaining growth of these mycobacteria.






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2004 - Indian Journal of Medical Microbiology
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