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Year : 1995  |  Volume : 13  |  Issue : 2  |  Page : 80-82

Diagnostic evaluation of iodine, giemsa and methyl green neutral red staining methods for defecting chlamydia trachomatis


Department of Microbiology, Government Medical College, Nagpur

Correspondence Address:
SN Shrikhande
Department of Microbiology, Government Medical College, Nagpur

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Source of Support: None, Conflict of Interest: None


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ABSTRACT: Although Chlamydia trachomatis has been identified as an important sexually transmitted agent, screening of patients for chlamydia is rarely performed due to the non-availability of accurate simple tests. This study evaluated the diagnostic accuracy of Iodine, Giemsa and Methyl Green Neutral Red (MGNR) staining methods for detecting C.trachomatis infection, by using ELISA as the 'Gold Standard'. The sensitivity and specificity of MGNR staining method were estimated to be 91.11 percent and 82.51percent respectively, which were significantly higher than those for Giemsa and Iodine staining methods and overall predictive accuracy was estimated to be 86.81 percent. Hence, MGNR staining method could be the method of choice among the staining methods for detecting C.trachomatis inclusions and can be recommended for routine screening.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04