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Year : 1992  |  Volume : 10  |  Issue : 1  |  Page : 56-59

Comparison Of Latex Agglutination Test With Elisa And Electron Microscopy For Detection Of Human Rotavirus In Stool



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N.T Gokhale


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A Commercial latex agglutination (LA) test was compared with ELISA and electron microscopy (EM) for detection of rotavirus antigen in 100 stool specimens of children with acute diarrhoea. Eighty-five stool specimens (85%) were either positive or negative with all the three tests used while 15 specimens (15%) gave contradictory results. Only 1 specimen was positive by LA test but not the ELISA or EM. The Sensitivity of LA test was 45.8 percent (11 of 24), and that of EM was 98.6 percent (75 of 76), and that of EM was 100 percent (76 of 76). The ELISA test detected more positive (24) specimens than the EM (14) or the LA test (12). The sensitivity of LA test was found to be low, but it is highly specific and rapid, hence may be useful in certain situations.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04