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Year : 1991  |  Volume : 9  |  Issue : 4  |  Page : 224-229

Inhibition Of Mycolic Acid Biosynthesis In Mycobacteria By Streptomycin And Isoniazid



Correspondence Address:
M. A Patil


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Source of Support: None, Conflict of Interest: None


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With an aim to create model systems and better understanding the mechanism of action of anti-mycobacterial drugs especially in pathogens like M.leprae, two cultivable Mycobacteria, M.gordonae a Mycobacterium whose mycolic and profile is similar to that of M.leprae and M.fortuitum a human pathogen, have been investigated. The effect of streptomycin was studied on M.gordonse whereas isoniazid was studied on M. fortuitum. The Mycobacteria grown in Sauton’s medium were treated with 0.5, 1, 2, 3, 4, mcg/ml concentrations of Streptomycin and Isoniazid during their mid logarithmic phase for 1, 2, 24, 48 hrs and then were harvested, The ‘bound lipid’ fraction comprising chiefly of mycolic acid was extracted and isolated from the cell wall structure by acid methanolysis and the methyl esters of mycolates were analysed by thin-layer chromatography using aluminum backed HP-TLC plates. Streptomycin was found to reduce methyl mycolate levels of M.gordonae at high concentrations (3,4 mcg/ml). Isoniazid exhibited its inhibitory action over mycolates of M.fortuitum at 0.5, 1.0 and 2.0 mcg/ml. The results of this study and our earlier reported observations show that mycolic acid biosynthesis is affected by various types of drugs eventhough the primary site of activity may be different for them. These observations may have therapeutic significance for developing model system to understand the effect of drugs on biosynthetic processes and degradation of cell wall skeleton on general in pathogenic Mycobacteria.






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