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Year : 1991  |  Volume : 9  |  Issue : 4  |  Page : 219-223

Detection Of Genomic Variation In Slow And Fast Growing Mycobacteria By Analysis Of restriction Fragment Length Polymorphism (RFLP) Of Ribosomal RNA Genes



Correspondence Address:
G. V Kanaujia


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Identifying and classifying pathogenic Mycobacteria is time consuming and remains a problem. We have earlier standardized techniques to study the variation in rRNA genes of Mycobacteria and have reported that restriction fragment analysis of rRNA genes of Mycobacteria could be useful for distinguishing some rapidly growing and slow growing Mycobacteria. In the present extended study, DNA was isolated from M.fortuitum, M.chelonei, M.avium, M.gordonae and M.kansasii. EAch DNA sample was cleaved with restriction endonclease EcoRI. After electrophoresis in gels, the DNA fragment containing rRNA gene sequences were transferred to nitrocellulose membranes and hybridized with -32p labeled Mycobacterial rRNA probe. The patterns of bands (the rDNA finger prints), which depended on Restriction Fragment Length Polymorphism (RFLP) of fragments containing rRNA genes, indicate that all these species could be distinguished from each other and patterns were found to be species specific. Results of this extended study show that this approach can be used for detection of genomic variation and rapid characterization of medically important Mycobacterial species.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

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