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Year : 1991  |  Volume : 9  |  Issue : 4  |  Page : 183-187

Antigenic Components Of Toxoplasma Gondii : Their Identification And Diagnostic Significance



Correspondence Address:
P Sharma


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Eight major fractions of soluble extract of Toxoplasma gondii (RH strain), obtained on Sephadex G-200 column, were identified evaluated for their diagnostic significance in patients with bad obstetrical history. These fractions were probed with two groups of serum samples, Group I having negative antitoxoplasma antibody titres and a Group II with high positive antibody titres as checked by indirect haemagglutination assay and indirect fluorescent antibody test. Only Fraction V did not react with sera of Group I by ELISA-IgG, while all other fractions and the soluble extract gave some false positive results. All fractions including fraction V reacted positively with Group II sera. On SDS-PAGE analysis, fraction V, showed a prominent band with a molecular weight of approximately 20 kilodaltons. Fraction V, therefore, seems to be the specific antigenic component for serodiagnosis of toxoplasmosis.






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2004 - Indian Journal of Medical Microbiology
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