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Year : 1991  |  Volume : 9  |  Issue : 3  |  Page : 166-168

Comparison Of Two Methods Of Decontamination Of Sputum For Isolation Of Tubercle Bacilli



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M. P Bansal


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Eighty one sputum specimens from cases of pulmonary tuberculosis on anti-tuberculosis treatment were cultured. Two decontamination techniques viz. Petroff’s method and Nassau’s swab method were used for all specimens before the culture. Culture positivity was 58% by Petroff’s and 60.50% by Nassau’s method. The growth of Mycobacterium tuberculosis after Nassau’s method was faster than that after Petroff’s method. Contamination of culture slopes was almost equal with both the decontamination techniques. Smear positive (direct smear of sputum by Z.N. Stain) but culture negative specimens were 21.1% All these results are discussed.






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2004 - Indian Journal of Medical Microbiology
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