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Year : 1991  |  Volume : 9  |  Issue : 3  |  Page : 156-161

Detection Of Circulating Parasite Antigen In Bancroftian Filariasis By Counter-Immunoelectrophoresis Using Antibodies To SD2-4 Fraction From Heterologous S. Digitata

Correspondence Address:
N Mallikarjun

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Source of Support: None, Conflict of Interest: None

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Antibodies raised against SD2-4 fraction of Setaria digitata (whole worm) extract, fractionated by DEAE-A50 column chromatography and antibodies to unfractionated whole worm antigens were used in counterimmunoelectrophoresis for the detection of circulating antigens in sera of bancroftian filariasis. While using SD2-4 antibodies, antigen could be detected in 13 of the 20 (65%) microfilaraemic carriers, 11 of 20 (55%) chronic cases and 4 of 20 (20%) endemic normals. No antigen could be detected in non endemic normal cases. On, the other hand, while using antibodies to whole worm extract antigen could be detected in 17 of 20 (85%) microfilaraemic carriers 15 of 20 (75%) chronic cases and 7 of 20 (35%) endemic normals. No antigen could be detected in non-endemic normal cases. Antibodies to whole worm antigen could detect circulating antigens in more number of cases of bancroftian filariasis.

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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04