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Year : 1990  |  Volume : 8  |  Issue : 2  |  Page : 40-43

Evaluation Of HSV-2 Bgl-II N Fragment



Correspondence Address:
J Kishore


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Source of Support: None, Conflict of Interest: None


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A recently developed sub-genomic DNA probe (7.2kb), HSV-2 Bgl-II N fragment cloned in PKC-7 plasmid has been evaluated in comparison to whole genomic HSV-2 DNA for specific detection of herpes simplex virus type-2 by filter hybridization. The probes were vector freed, nick-translated using 32pdCTP to a specific activity of 0.5-1 x 108 cpm/ug DNA. The whole gonomic HSV-2 DNA hybridized under stringent conditions with mock-infected cells and cells infected with other viruses as reveled by autoadiography. In contrast HSV-2 Bgl-II N fragment probe hybridized specifically only with HSV-2 infected vero cells and could detect 103 or more of such cells. Storage of the infected cells prior to hybridization at 4 C or –70 C for 24 hours did not alter the sensitivity or specificity of detection. Thus HSV-2 Bgl II N fragment can be used as rapid, specific and sensitive probe for the detection of herpes simplex virus type-2 infected cells.






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2004 - Indian Journal of Medical Microbiology
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