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Year : 1990  |  Volume : 8  |  Issue : 1  |  Page : 17-22

Analysis Of Nonstructural Polypeptides Of Japanese Encephalitis And West Nile Viruses



Correspondence Address:
K Banerjee


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Source of Support: None, Conflict of Interest: None


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Japanese encephalitis (JE) and West (WN) viruses were grown on porcine stable (PS) cells and radiolabelled with tritiated amino acids and analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Two large molecular weight polypeptides were detected in cells infected with JE virus. One was of molecular weight 324000 and was detected during early phase of JE virus replication, (6-10 hours post Infection). Another polypeptide had molecular weight 174000 and was detected during late phase of JE virus replication (after 17 hours of post infection). The nonstructural polypeptide P71 of JE and WN viruses was found to differ in size. The P71 polypeptide of JE virus was of larger molecular weight as compared to that of WN virus.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

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