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Year : 1989  |  Volume : 7  |  Issue : 3  |  Page : 98-104

Enzyme-Linked Immunosorbent Assay For IgG Antibody To Tuberculin Purified Protein Derivative In Cerebrospinal Fluid Specimens Of Patients With Tuberclous Meningitis



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A Mathai


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Tuberculin purified protein derivative (PPD) was prepared in this laboratory from H37Ra strain of M. tuberculosis. Using this antigen, non-competitive Enzyme-linked immunosorbent assay (ELISA) was carried out to detect lgG antibody in cerebrospinal fluid (CSF) specimens from forty patients with clinical diagnosis of tuberculous meningitis (TBM) and the data was compared with ten culture positive patients (positive control) of TBM. CSF specimens from fifty patients from non-tuberculosis neurological disease formed the control group in this study. The antibody titre did not depend upon lgG concentration in CSF, tuberculin reactor status or duration of chemotherapy in patients with TBM. The geometric mean antibody titre for tuberculous and non-tuberculous subjects were 195.5 and 20.5 respectively. At a dilution end-point 1:80 the assay had a sensitivity of 75% and specificity of 84.0%. However at dilution end-point 1:160 the specificity of the assay could be increased to 100%, but sensitivity of the assay was 70%. The non-competitive ELISA described in this communication has potential diagnostic application in patients with TBM in whom repeated cultures in CSF specimens fail to demonstrate the causative agent M. tuberculosis.






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2004 - Indian Journal of Medical Microbiology
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