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Year : 1987  |  Volume : 5  |  Issue : 2  |  Page : 85-99

Glutaraldehyde Inactivated Pertussis Vaccine A Safe, Potent And Stable Vaccine



Correspondence Address:
R. K Gupta


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Source of Support: None, Conflict of Interest: None


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The whole cell pertussis vaccine was prepared using glutaraldehyde as an inactivating agent and evaluated with the routinely produced heat inactivated pertussis vaccine (HIPV) for potency, toxicity and stability. Five batches of each of glutaraldehyde inactivated pertussis vaccine (GIPV) and HIPV were prepared for each of B. pertussis strains 134 and 509. The potency of GIPV was very similar to that of HIPV showing no statistically significant differences (p>0.05). The GIPV had about 3 to 8 times less histamine sensitizing activity than HIPV. The mice injected with GIPV showed slightly higher weight gains than those injected with HIPV showing ‘lesser toxicity of GIPV in monse weight gain test. In the innocuity test also, the adsorbed diphtheria-pertussis-tetanus (DPT) vaccine containing GIPV was safer vaccine than the DPT vaccine containing HIPV. The stability of GIPV was similar to that of HIPV. Both the vaccines were stable in potency at 4-8C and 25C for three months while these lost about 60% of their potencies at 37C in 3 months. In this study it has been observed that glutaraldehyde under the prescribed conditions is a better inactivating agent than heat for preparation of a safe, potent and stable pertussis vaccine.






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