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Year : 1986  |  Volume : 4  |  Issue : 3  |  Page : 177-180

Evaluation Of Homogenising Substances In Estimating The Viability Of Tubercle Bacilli


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C. N Paramasivan


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A randomized, concurrent comparison of the following methods for the processing of sputum specimens from tuberculous patients for estimation the viable population of M. tuberculosis, was undertaken; (1) sputolysin + 1% cetrimide; (2) bromhexine + 1% cetrimide; (3) standard petroff’s. The mean log viable count was 5.64 by the sputolysin, 5.21 by bromhexine and 5.35 by the petroff’s method. The difference between the sputolysin and petroff’s methods was statistically significant (P=0.01). The bromhexine method, which had a value similar to Petroff’s, can be used as an alternative method.






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2004 - Indian Journal of Medical Microbiology
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